Available Technologies


Project TitleTetrazine-Trans-Cyclooctene Ligation as the Basis of PET/SPECT Probes and Radiotherapies
Track Code2011-002
Short Description

The innovation is a new class of ligation reaction that proceeds with exceptionally high reaction rates.  The reaction involves the cycloaddition of 1,2,4,5-tetrazene derivatives with dienophiles.  The reaction tolerates a broad rate of functionality, including thiols and primary amines, and the reaction proceeds in high yield in organic solvents and water.  Examples of tetrazene ligation reactions are demonstrated in biological media and in cell lysate.  The selectivity and aqueous compatibility of the reaction make it suitable for numerous in vivo and in vitro applications, including the ligation reactions of small molecules, peptides, proteins, oligonucleotides, other types of polymers, nanoparticles, and on surfaces (e.g. glass slides, gold).  Also described are new methods for producing tetrazines, cyclopropene derivatives, and trans-cyclooctene derivatives.  

Tagstetrazene, ligation, PET/SPECT, probes, radiotherapies
Posted DateOct 21, 2011 10:01 AM


  • Utilizes tetrazines for bioorthogonal ligations.
  • Exceptionally fast reaction rates, high yielding specific reactivity, broad functional group tolerance, suitable for modifying proteins, no offensive byproducts.


  • Real-time in vivo imaging
  • Radiation therapy


Denise M. Bierlein
University of Delaware
Office of Economic Innovation and Partnerships
Licensing Analysis
Telephone:  (302) 831-4005
Email:  deniseb@udel.edu

Intellectual Property

Patent Number Issue Date Type Country of Filing
Patent Pending None Utility Not Applicable (PCT App)