Projects Sharing Researchers
- Very-High Efficiency Solar Cell (VHESC)
- One-Pot Synthesis of 18F FMAU
- Ultrafast Crosslinking Reactions for Creating Hydrogels for Applications in in vitro Culture and Regenerative Medicine
- Trans-Cycloheptene and Hetero-Trans-Cycloheptenes for Bioorthogonal Labeling
- Highly Reactive Trans-Cyclooctenes For Radiochemical Labeling
|Project Title||Tetrazine-Trans-Cyclooctene Ligation as the Basis of PET/SPECT Probes and Radiotherapies|
The innovation is a new class of ligation reaction that proceeds with exceptionally high reaction rates. The reaction involves the cycloaddition of 1,2,4,5-tetrazene derivatives with dienophiles. The reaction tolerates a broad rate of functionality, including thiols and primary amines, and the reaction proceeds in high yield in organic solvents and water. Examples of tetrazene ligation reactions are demonstrated in biological media and in cell lysate. The selectivity and aqueous compatibility of the reaction make it suitable for numerous in vivo and in vitro applications, including the ligation reactions of small molecules, peptides, proteins, oligonucleotides, other types of polymers, nanoparticles, and on surfaces (e.g. glass slides, gold). Also described are new methods for producing tetrazines, cyclopropene derivatives, and trans-cyclooctene derivatives.
|Tags||tetrazene, ligation, PET/SPECT, probes, radiotherapies|
|Posted Date||Oct 21, 2011 10:01 AM|
- Utilizes tetrazines for bioorthogonal ligations.
- Exceptionally fast reaction rates, high yielding specific reactivity, broad functional group tolerance, suitable for modifying proteins, no offensive byproducts.
University of Delaware
Office of Economic Innovation and Partnerships
Telephone: (302) 831-4005
|Patent Number||Issue Date||Type||Country of Filing|
|Patent Pending||None||Utility||Not Applicable (PCT App)|